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dmso treatment  (ATCC)


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    Structured Review

    ATCC dmso treatment
    Transcriptome sequencing analysis of C. albicans treated <t>with</t> <t>BD11</t> . (A) Volcano plots display comparisons of C. albicans ATCC 10231 treated with <t>DMSO</t> or BD11 (6.25 µg/mL) for 8h. The two vertical dashed lines in the graph indicate the 2-fold expression difference threshold and the horizontal dashed line represents the P -value threshold of 0.05. Red dots indicate up-regulated genes, blue dots indicate down-regulated genes, and grey dots represent genes with no significant difference in expression. (B) The most significantly enriched 20 GO terms for DEGs. Circle size represents the gene number. The FDR (false discovery rate, corrected P -value) generally ranges from 0 to 1, with values closer to zero indicating more significant enrichment. (C) Top 30 KEGG pathways for DEGs. The horizontal axis represents the pathway name, and the vertical axis represents the −log 10 of the P -value for each pathway's enrichment. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)
    Dmso Treatment, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 2103 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Images

    1) Product Images from "Boronic acid derivatives inhibit Candida albicans growth by compromising energy metabolism"

    Article Title: Boronic acid derivatives inhibit Candida albicans growth by compromising energy metabolism

    Journal: Journal of Advanced Research

    doi: 10.1016/j.jare.2025.07.044

    Transcriptome sequencing analysis of C. albicans treated with BD11 . (A) Volcano plots display comparisons of C. albicans ATCC 10231 treated with DMSO or BD11 (6.25 µg/mL) for 8h. The two vertical dashed lines in the graph indicate the 2-fold expression difference threshold and the horizontal dashed line represents the P -value threshold of 0.05. Red dots indicate up-regulated genes, blue dots indicate down-regulated genes, and grey dots represent genes with no significant difference in expression. (B) The most significantly enriched 20 GO terms for DEGs. Circle size represents the gene number. The FDR (false discovery rate, corrected P -value) generally ranges from 0 to 1, with values closer to zero indicating more significant enrichment. (C) Top 30 KEGG pathways for DEGs. The horizontal axis represents the pathway name, and the vertical axis represents the −log 10 of the P -value for each pathway's enrichment. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)
    Figure Legend Snippet: Transcriptome sequencing analysis of C. albicans treated with BD11 . (A) Volcano plots display comparisons of C. albicans ATCC 10231 treated with DMSO or BD11 (6.25 µg/mL) for 8h. The two vertical dashed lines in the graph indicate the 2-fold expression difference threshold and the horizontal dashed line represents the P -value threshold of 0.05. Red dots indicate up-regulated genes, blue dots indicate down-regulated genes, and grey dots represent genes with no significant difference in expression. (B) The most significantly enriched 20 GO terms for DEGs. Circle size represents the gene number. The FDR (false discovery rate, corrected P -value) generally ranges from 0 to 1, with values closer to zero indicating more significant enrichment. (C) Top 30 KEGG pathways for DEGs. The horizontal axis represents the pathway name, and the vertical axis represents the −log 10 of the P -value for each pathway's enrichment. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)

    Techniques Used: Sequencing, Expressing

    BD11 impairs cell wall integrity and perturbs energy metabolism in C. albicans . (A) Representative TEM images of C. albicans ATCC 10231 and BD11 -treated C. albicans ATCC 10231 (n = 3). Scale bar, 500 nm. The structures indicated by the arrows are mitochondria. (B) Calcofluor white staining of C. albicans ATCC 10231 treated with DMSO or BD11 (0.78, 1.56, and 3.125 µg/mL) at 30 °C for 6 h (n = 3). (C) Intracellular ATP content in C. albicans ATCC 10231 treated with DMSO or BD11 (0.78, 1.56, and 3.125 µg/mL) at 30 °C for 6h (n = 4). (D) The intracellular SDH activity in C. albicans ATCC 10231 treated with DMSO or BD11 (0.78, 1.56, and 3.125 µg/mL) at 30 °C for 6h (n = 4). The data are shown as the mean ± SEM. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001 vs DMSO.
    Figure Legend Snippet: BD11 impairs cell wall integrity and perturbs energy metabolism in C. albicans . (A) Representative TEM images of C. albicans ATCC 10231 and BD11 -treated C. albicans ATCC 10231 (n = 3). Scale bar, 500 nm. The structures indicated by the arrows are mitochondria. (B) Calcofluor white staining of C. albicans ATCC 10231 treated with DMSO or BD11 (0.78, 1.56, and 3.125 µg/mL) at 30 °C for 6 h (n = 3). (C) Intracellular ATP content in C. albicans ATCC 10231 treated with DMSO or BD11 (0.78, 1.56, and 3.125 µg/mL) at 30 °C for 6h (n = 4). (D) The intracellular SDH activity in C. albicans ATCC 10231 treated with DMSO or BD11 (0.78, 1.56, and 3.125 µg/mL) at 30 °C for 6h (n = 4). The data are shown as the mean ± SEM. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001 vs DMSO.

    Techniques Used: Staining, Activity Assay

    Compound BD11 inhibits C. albicans filamentation and biofilm formation. (A) Representative images of C. albicans ATCC 10231 were grown at 30 °C or 42°C for 6 h in the absence or presence of a gradient of BD11 (n = 3). (B) Representative images of C. albicans ATCC 10231 were grown at 42°C for 6 h or 12 h in the absence or presence of a gradient of BD11 (n = 3). (C–D) Biofilms formed by C. albicans ATCC 10231 (C) or C. albicans CMCC 98001 (D) were grown at 37 °C for 24 h in the absence or presence of BD11 as indicated. Crystal violet staining was used for the quantitative analysis of biofilms (n = 3). (E) Intracellular ATP content in C. albicans ATCC 10231 treated with DMSO or BD11 (0.78, 1.56, and 3.125 µg/mL) at 42 °C for 6 h (n = 5). (F) qPCR analysis of mRNA levels of RAS1 (n = 4) , TPK2 (n = 3) and EFG1 (n = 3) in C. albicans ATCC 10231 treated with DMSO or BD11 (0.78, 1.56, and 3.125 µg/mL) at 42 °C for 6 h. The data are shown as the mean ± SEM. * P < 0.05, ** P < 0.01, **** P < 0.0001 vs. DMSO. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)
    Figure Legend Snippet: Compound BD11 inhibits C. albicans filamentation and biofilm formation. (A) Representative images of C. albicans ATCC 10231 were grown at 30 °C or 42°C for 6 h in the absence or presence of a gradient of BD11 (n = 3). (B) Representative images of C. albicans ATCC 10231 were grown at 42°C for 6 h or 12 h in the absence or presence of a gradient of BD11 (n = 3). (C–D) Biofilms formed by C. albicans ATCC 10231 (C) or C. albicans CMCC 98001 (D) were grown at 37 °C for 24 h in the absence or presence of BD11 as indicated. Crystal violet staining was used for the quantitative analysis of biofilms (n = 3). (E) Intracellular ATP content in C. albicans ATCC 10231 treated with DMSO or BD11 (0.78, 1.56, and 3.125 µg/mL) at 42 °C for 6 h (n = 5). (F) qPCR analysis of mRNA levels of RAS1 (n = 4) , TPK2 (n = 3) and EFG1 (n = 3) in C. albicans ATCC 10231 treated with DMSO or BD11 (0.78, 1.56, and 3.125 µg/mL) at 42 °C for 6 h. The data are shown as the mean ± SEM. * P < 0.05, ** P < 0.01, **** P < 0.0001 vs. DMSO. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)

    Techniques Used: Staining



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    Transcriptome sequencing analysis of C. albicans treated <t>with</t> <t>BD11</t> . (A) Volcano plots display comparisons of C. albicans ATCC 10231 treated with <t>DMSO</t> or BD11 (6.25 µg/mL) for 8h. The two vertical dashed lines in the graph indicate the 2-fold expression difference threshold and the horizontal dashed line represents the P -value threshold of 0.05. Red dots indicate up-regulated genes, blue dots indicate down-regulated genes, and grey dots represent genes with no significant difference in expression. (B) The most significantly enriched 20 GO terms for DEGs. Circle size represents the gene number. The FDR (false discovery rate, corrected P -value) generally ranges from 0 to 1, with values closer to zero indicating more significant enrichment. (C) Top 30 KEGG pathways for DEGs. The horizontal axis represents the pathway name, and the vertical axis represents the −log 10 of the P -value for each pathway's enrichment. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)
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    Image Search Results


    Transcriptome sequencing analysis of C. albicans treated with BD11 . (A) Volcano plots display comparisons of C. albicans ATCC 10231 treated with DMSO or BD11 (6.25 µg/mL) for 8h. The two vertical dashed lines in the graph indicate the 2-fold expression difference threshold and the horizontal dashed line represents the P -value threshold of 0.05. Red dots indicate up-regulated genes, blue dots indicate down-regulated genes, and grey dots represent genes with no significant difference in expression. (B) The most significantly enriched 20 GO terms for DEGs. Circle size represents the gene number. The FDR (false discovery rate, corrected P -value) generally ranges from 0 to 1, with values closer to zero indicating more significant enrichment. (C) Top 30 KEGG pathways for DEGs. The horizontal axis represents the pathway name, and the vertical axis represents the −log 10 of the P -value for each pathway's enrichment. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)

    Journal: Journal of Advanced Research

    Article Title: Boronic acid derivatives inhibit Candida albicans growth by compromising energy metabolism

    doi: 10.1016/j.jare.2025.07.044

    Figure Lengend Snippet: Transcriptome sequencing analysis of C. albicans treated with BD11 . (A) Volcano plots display comparisons of C. albicans ATCC 10231 treated with DMSO or BD11 (6.25 µg/mL) for 8h. The two vertical dashed lines in the graph indicate the 2-fold expression difference threshold and the horizontal dashed line represents the P -value threshold of 0.05. Red dots indicate up-regulated genes, blue dots indicate down-regulated genes, and grey dots represent genes with no significant difference in expression. (B) The most significantly enriched 20 GO terms for DEGs. Circle size represents the gene number. The FDR (false discovery rate, corrected P -value) generally ranges from 0 to 1, with values closer to zero indicating more significant enrichment. (C) Top 30 KEGG pathways for DEGs. The horizontal axis represents the pathway name, and the vertical axis represents the −log 10 of the P -value for each pathway's enrichment. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)

    Article Snippet: BD11 or DMSO treatment of C. albicans ATCC 10231 samples was performed as described in the electron microscopy methods section.

    Techniques: Sequencing, Expressing

    BD11 impairs cell wall integrity and perturbs energy metabolism in C. albicans . (A) Representative TEM images of C. albicans ATCC 10231 and BD11 -treated C. albicans ATCC 10231 (n = 3). Scale bar, 500 nm. The structures indicated by the arrows are mitochondria. (B) Calcofluor white staining of C. albicans ATCC 10231 treated with DMSO or BD11 (0.78, 1.56, and 3.125 µg/mL) at 30 °C for 6 h (n = 3). (C) Intracellular ATP content in C. albicans ATCC 10231 treated with DMSO or BD11 (0.78, 1.56, and 3.125 µg/mL) at 30 °C for 6h (n = 4). (D) The intracellular SDH activity in C. albicans ATCC 10231 treated with DMSO or BD11 (0.78, 1.56, and 3.125 µg/mL) at 30 °C for 6h (n = 4). The data are shown as the mean ± SEM. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001 vs DMSO.

    Journal: Journal of Advanced Research

    Article Title: Boronic acid derivatives inhibit Candida albicans growth by compromising energy metabolism

    doi: 10.1016/j.jare.2025.07.044

    Figure Lengend Snippet: BD11 impairs cell wall integrity and perturbs energy metabolism in C. albicans . (A) Representative TEM images of C. albicans ATCC 10231 and BD11 -treated C. albicans ATCC 10231 (n = 3). Scale bar, 500 nm. The structures indicated by the arrows are mitochondria. (B) Calcofluor white staining of C. albicans ATCC 10231 treated with DMSO or BD11 (0.78, 1.56, and 3.125 µg/mL) at 30 °C for 6 h (n = 3). (C) Intracellular ATP content in C. albicans ATCC 10231 treated with DMSO or BD11 (0.78, 1.56, and 3.125 µg/mL) at 30 °C for 6h (n = 4). (D) The intracellular SDH activity in C. albicans ATCC 10231 treated with DMSO or BD11 (0.78, 1.56, and 3.125 µg/mL) at 30 °C for 6h (n = 4). The data are shown as the mean ± SEM. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001 vs DMSO.

    Article Snippet: BD11 or DMSO treatment of C. albicans ATCC 10231 samples was performed as described in the electron microscopy methods section.

    Techniques: Staining, Activity Assay

    Compound BD11 inhibits C. albicans filamentation and biofilm formation. (A) Representative images of C. albicans ATCC 10231 were grown at 30 °C or 42°C for 6 h in the absence or presence of a gradient of BD11 (n = 3). (B) Representative images of C. albicans ATCC 10231 were grown at 42°C for 6 h or 12 h in the absence or presence of a gradient of BD11 (n = 3). (C–D) Biofilms formed by C. albicans ATCC 10231 (C) or C. albicans CMCC 98001 (D) were grown at 37 °C for 24 h in the absence or presence of BD11 as indicated. Crystal violet staining was used for the quantitative analysis of biofilms (n = 3). (E) Intracellular ATP content in C. albicans ATCC 10231 treated with DMSO or BD11 (0.78, 1.56, and 3.125 µg/mL) at 42 °C for 6 h (n = 5). (F) qPCR analysis of mRNA levels of RAS1 (n = 4) , TPK2 (n = 3) and EFG1 (n = 3) in C. albicans ATCC 10231 treated with DMSO or BD11 (0.78, 1.56, and 3.125 µg/mL) at 42 °C for 6 h. The data are shown as the mean ± SEM. * P < 0.05, ** P < 0.01, **** P < 0.0001 vs. DMSO. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)

    Journal: Journal of Advanced Research

    Article Title: Boronic acid derivatives inhibit Candida albicans growth by compromising energy metabolism

    doi: 10.1016/j.jare.2025.07.044

    Figure Lengend Snippet: Compound BD11 inhibits C. albicans filamentation and biofilm formation. (A) Representative images of C. albicans ATCC 10231 were grown at 30 °C or 42°C for 6 h in the absence or presence of a gradient of BD11 (n = 3). (B) Representative images of C. albicans ATCC 10231 were grown at 42°C for 6 h or 12 h in the absence or presence of a gradient of BD11 (n = 3). (C–D) Biofilms formed by C. albicans ATCC 10231 (C) or C. albicans CMCC 98001 (D) were grown at 37 °C for 24 h in the absence or presence of BD11 as indicated. Crystal violet staining was used for the quantitative analysis of biofilms (n = 3). (E) Intracellular ATP content in C. albicans ATCC 10231 treated with DMSO or BD11 (0.78, 1.56, and 3.125 µg/mL) at 42 °C for 6 h (n = 5). (F) qPCR analysis of mRNA levels of RAS1 (n = 4) , TPK2 (n = 3) and EFG1 (n = 3) in C. albicans ATCC 10231 treated with DMSO or BD11 (0.78, 1.56, and 3.125 µg/mL) at 42 °C for 6 h. The data are shown as the mean ± SEM. * P < 0.05, ** P < 0.01, **** P < 0.0001 vs. DMSO. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)

    Article Snippet: BD11 or DMSO treatment of C. albicans ATCC 10231 samples was performed as described in the electron microscopy methods section.

    Techniques: Staining